314 bm 010 Search Results


bmp4  (R&D Systems)
96
R&D Systems bmp4
A Immunofluorescence for γH2a.x and Troponin shows that BMP2 or BMP7 rescue cultured primary neonatal mouse CMs from HU-induced replication stress. Data points represent the fraction of γH2a.x+ CMs per well. n E = 2, n wells = 10 media, 10 BMP7, 9 BMP2, 12 media (HU), 9 BMP7 (HU), 10 BMP2 (HU). n C = 2795 media, 2658 BMP7, 2106 BMP2, 3593 media (HU), 2704 BMP7 (HU), 2533 BMP2 (HU). Scale bar, 20 μm. B The p38 MAPK inhibitor SB202190 does not protect CMs from HU-induced replication stress. n E = 2, n wells = 6 media, 4 p38i, 8 media (HU), 4 p38i (HU), n C = 1384 media, 765 p38i, 1937 media (HU), 876 p38i (HU). The observed relative difference between HU (media) and HU (p38i) groups is 11%, the calculated smallest significant difference 36%, which is smaller than the effect size between the HU (media) and HU (BMP7) groups of 44% (from Fig. 7A). We conclude that this experiment had enough power to detect effects of that magnitude. C Immunofluorescence for γH2a.x shows that pretreatment with BMP2 and <t>BMP4</t> protects U2OS cells from HU-induced replication stress. Plots show integrated intensity of γH2a.x nuclear levels. n E = 1, n wells = 3 for each treatment. Scale bar, 20 μm. D Immunofluorescence for γH2a.x and Phalloidin in human primary neonatal foreskin dermal fibroblasts shows that pre-treatment with BMP2 and BMP4 alleviates HU-induced replication stress. Data points represent quantification of γH2a.x nuclear levels. n E = 1, n C = 14 BSA, 11 BMP2 + 4, 14 BSA (HU), 22 BMP2 + 4 (HU). Scale bar, 20 μm. E Pre-treatment with BMP2 rescues survival and proliferative capacity of primary human bone-marrow derived HSPCs treated with HU. Data represent the colony forming units observed in HSPCs isolated from 3 individual donors normalized to BSA-treated controls. n E = 5, n donors = 3, n plates = 2 for donors 1 and 2; 1 for donor 3. A – C , E Ordinary one-way ANOVA with Bonferroni correction; D Kruskal-Wallis followed by Dunn´s correction. A – E Data are presented as mean values. Error bars, CI 95%. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).
Bmp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/314+bm+010/Recombinant+Human%2FMouse%2FRat+BMP-2+Protein/pmc11832743-491-47-48
Average 96 stars, based on 1 article reviews
bmp4 - by Bioz Stars, 2026-09
96/100 stars
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314 bm 010  (R&D Systems)
94
R&D Systems 314 bm 010
A Immunofluorescence for γH2a.x and Troponin shows that BMP2 or BMP7 rescue cultured primary neonatal mouse CMs from HU-induced replication stress. Data points represent the fraction of γH2a.x+ CMs per well. n E = 2, n wells = 10 media, 10 BMP7, 9 BMP2, 12 media (HU), 9 BMP7 (HU), 10 BMP2 (HU). n C = 2795 media, 2658 BMP7, 2106 BMP2, 3593 media (HU), 2704 BMP7 (HU), 2533 BMP2 (HU). Scale bar, 20 μm. B The p38 MAPK inhibitor SB202190 does not protect CMs from HU-induced replication stress. n E = 2, n wells = 6 media, 4 p38i, 8 media (HU), 4 p38i (HU), n C = 1384 media, 765 p38i, 1937 media (HU), 876 p38i (HU). The observed relative difference between HU (media) and HU (p38i) groups is 11%, the calculated smallest significant difference 36%, which is smaller than the effect size between the HU (media) and HU (BMP7) groups of 44% (from Fig. 7A). We conclude that this experiment had enough power to detect effects of that magnitude. C Immunofluorescence for γH2a.x shows that pretreatment with BMP2 and <t>BMP4</t> protects U2OS cells from HU-induced replication stress. Plots show integrated intensity of γH2a.x nuclear levels. n E = 1, n wells = 3 for each treatment. Scale bar, 20 μm. D Immunofluorescence for γH2a.x and Phalloidin in human primary neonatal foreskin dermal fibroblasts shows that pre-treatment with BMP2 and BMP4 alleviates HU-induced replication stress. Data points represent quantification of γH2a.x nuclear levels. n E = 1, n C = 14 BSA, 11 BMP2 + 4, 14 BSA (HU), 22 BMP2 + 4 (HU). Scale bar, 20 μm. E Pre-treatment with BMP2 rescues survival and proliferative capacity of primary human bone-marrow derived HSPCs treated with HU. Data represent the colony forming units observed in HSPCs isolated from 3 individual donors normalized to BSA-treated controls. n E = 5, n donors = 3, n plates = 2 for donors 1 and 2; 1 for donor 3. A – C , E Ordinary one-way ANOVA with Bonferroni correction; D Kruskal-Wallis followed by Dunn´s correction. A – E Data are presented as mean values. Error bars, CI 95%. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).
314 Bm 010, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/314+bm+010/Recombinant+Mouse+BMP-6+Protein/pmc03177035-326-10-7
Average 94 stars, based on 1 article reviews
314 bm 010 - by Bioz Stars, 2026-09
94/100 stars
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recombinant human bmp 4 protein  (R&D Systems)
N/A
The Recombinant Human BMP 4 Protein from R D Systems is derived from NS0 The Recombinant Human BMP 4 Protein has been validated for the following applications Bioactivity
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recombinant human bmp 4 protein cf  (R&D Systems)
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The Recombinant Human BMP 4 Protein from R D Systems is derived from NS0 The Recombinant Human BMP 4 Protein has been validated for the following applications Bioactivity
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recombinant human bmp 4 gmp protein cf  (R&D Systems)
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The Recombinant Human BMP 4 GMP Protein from R D Systems is derived from NS0 The Recombinant Human BMP 4 GMP Protein has been validated for the following applications Bioactivity
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Image Search Results


A Immunofluorescence for γH2a.x and Troponin shows that BMP2 or BMP7 rescue cultured primary neonatal mouse CMs from HU-induced replication stress. Data points represent the fraction of γH2a.x+ CMs per well. n E = 2, n wells = 10 media, 10 BMP7, 9 BMP2, 12 media (HU), 9 BMP7 (HU), 10 BMP2 (HU). n C = 2795 media, 2658 BMP7, 2106 BMP2, 3593 media (HU), 2704 BMP7 (HU), 2533 BMP2 (HU). Scale bar, 20 μm. B The p38 MAPK inhibitor SB202190 does not protect CMs from HU-induced replication stress. n E = 2, n wells = 6 media, 4 p38i, 8 media (HU), 4 p38i (HU), n C = 1384 media, 765 p38i, 1937 media (HU), 876 p38i (HU). The observed relative difference between HU (media) and HU (p38i) groups is 11%, the calculated smallest significant difference 36%, which is smaller than the effect size between the HU (media) and HU (BMP7) groups of 44% (from Fig. 7A). We conclude that this experiment had enough power to detect effects of that magnitude. C Immunofluorescence for γH2a.x shows that pretreatment with BMP2 and BMP4 protects U2OS cells from HU-induced replication stress. Plots show integrated intensity of γH2a.x nuclear levels. n E = 1, n wells = 3 for each treatment. Scale bar, 20 μm. D Immunofluorescence for γH2a.x and Phalloidin in human primary neonatal foreskin dermal fibroblasts shows that pre-treatment with BMP2 and BMP4 alleviates HU-induced replication stress. Data points represent quantification of γH2a.x nuclear levels. n E = 1, n C = 14 BSA, 11 BMP2 + 4, 14 BSA (HU), 22 BMP2 + 4 (HU). Scale bar, 20 μm. E Pre-treatment with BMP2 rescues survival and proliferative capacity of primary human bone-marrow derived HSPCs treated with HU. Data represent the colony forming units observed in HSPCs isolated from 3 individual donors normalized to BSA-treated controls. n E = 5, n donors = 3, n plates = 2 for donors 1 and 2; 1 for donor 3. A – C , E Ordinary one-way ANOVA with Bonferroni correction; D Kruskal-Wallis followed by Dunn´s correction. A – E Data are presented as mean values. Error bars, CI 95%. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).

Journal: Nature Communications

Article Title: BMP signaling promotes zebrafish heart regeneration via alleviation of replication stress

doi: 10.1038/s41467-025-56993-6

Figure Lengend Snippet: A Immunofluorescence for γH2a.x and Troponin shows that BMP2 or BMP7 rescue cultured primary neonatal mouse CMs from HU-induced replication stress. Data points represent the fraction of γH2a.x+ CMs per well. n E = 2, n wells = 10 media, 10 BMP7, 9 BMP2, 12 media (HU), 9 BMP7 (HU), 10 BMP2 (HU). n C = 2795 media, 2658 BMP7, 2106 BMP2, 3593 media (HU), 2704 BMP7 (HU), 2533 BMP2 (HU). Scale bar, 20 μm. B The p38 MAPK inhibitor SB202190 does not protect CMs from HU-induced replication stress. n E = 2, n wells = 6 media, 4 p38i, 8 media (HU), 4 p38i (HU), n C = 1384 media, 765 p38i, 1937 media (HU), 876 p38i (HU). The observed relative difference between HU (media) and HU (p38i) groups is 11%, the calculated smallest significant difference 36%, which is smaller than the effect size between the HU (media) and HU (BMP7) groups of 44% (from Fig. 7A). We conclude that this experiment had enough power to detect effects of that magnitude. C Immunofluorescence for γH2a.x shows that pretreatment with BMP2 and BMP4 protects U2OS cells from HU-induced replication stress. Plots show integrated intensity of γH2a.x nuclear levels. n E = 1, n wells = 3 for each treatment. Scale bar, 20 μm. D Immunofluorescence for γH2a.x and Phalloidin in human primary neonatal foreskin dermal fibroblasts shows that pre-treatment with BMP2 and BMP4 alleviates HU-induced replication stress. Data points represent quantification of γH2a.x nuclear levels. n E = 1, n C = 14 BSA, 11 BMP2 + 4, 14 BSA (HU), 22 BMP2 + 4 (HU). Scale bar, 20 μm. E Pre-treatment with BMP2 rescues survival and proliferative capacity of primary human bone-marrow derived HSPCs treated with HU. Data represent the colony forming units observed in HSPCs isolated from 3 individual donors normalized to BSA-treated controls. n E = 5, n donors = 3, n plates = 2 for donors 1 and 2; 1 for donor 3. A – C , E Ordinary one-way ANOVA with Bonferroni correction; D Kruskal-Wallis followed by Dunn´s correction. A – E Data are presented as mean values. Error bars, CI 95%. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).

Article Snippet: HSPCs were cultured for 24 h at 37 °C, 5% CO 2 and ambient O 2 in serum free StemSpanTM SFEM medium (STEMCELL Technologies) supplemented with 1% Penicillin/Streptomycin (Gibco by Thermo Fisher Scientific) and 1% StemSpanTM CC100 (STEMCELL Technologies,) before treatment with either 50 ng/ml BMP2 or BMP4 (R&D Systems #355-BM-010 and #314-BP-010) solved in 0.1% BSA (Sigma-Aldrich by Merck) for 48 h.

Techniques: Immunofluorescence, Cell Culture, Derivative Assay, Isolation

A DNA fiber spreading assays show that treatment with either BMP2 or BMP4 ligands for 48 h increases the speed of replication fork progression measured by track length of either CldU (green) or IdU (red) in human cord blood HSPCs. Data points represent length of individual CldU or IdU tracks. n E = 3, n fibers = 670 per treatment. B Treatment with BMP4 increases replication fork progression in U2OS cells, while BMP2 does not have a significant effect. n E = 2, n fibers = 250 per treatment. C Pre-treatment with BMP2 or BMP4 ligands increases the fraction of replication forks that restart after HU-mediated stalling in U2OS cells. Fork arrest is indicated by tracks that are only labeled by CldU, restarted forks by CldU tracks that are followed by IdU. Data points represent fraction of restarted forks out of all analyzed forks. n E = 2, n fibers = 600 per treatment. A – C Data are presented as mean values. Error bars, CI 95%. Kruskal-Wallis followed by Dunn´s correction. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).

Journal: Nature Communications

Article Title: BMP signaling promotes zebrafish heart regeneration via alleviation of replication stress

doi: 10.1038/s41467-025-56993-6

Figure Lengend Snippet: A DNA fiber spreading assays show that treatment with either BMP2 or BMP4 ligands for 48 h increases the speed of replication fork progression measured by track length of either CldU (green) or IdU (red) in human cord blood HSPCs. Data points represent length of individual CldU or IdU tracks. n E = 3, n fibers = 670 per treatment. B Treatment with BMP4 increases replication fork progression in U2OS cells, while BMP2 does not have a significant effect. n E = 2, n fibers = 250 per treatment. C Pre-treatment with BMP2 or BMP4 ligands increases the fraction of replication forks that restart after HU-mediated stalling in U2OS cells. Fork arrest is indicated by tracks that are only labeled by CldU, restarted forks by CldU tracks that are followed by IdU. Data points represent fraction of restarted forks out of all analyzed forks. n E = 2, n fibers = 600 per treatment. A – C Data are presented as mean values. Error bars, CI 95%. Kruskal-Wallis followed by Dunn´s correction. Source data are provided in the Source Data file. Some elements were created in Biorender (Agreement number: CH27UPPBB6; Posadas, (2025) https://BioRender.com/n30v908 ).

Article Snippet: HSPCs were cultured for 24 h at 37 °C, 5% CO 2 and ambient O 2 in serum free StemSpanTM SFEM medium (STEMCELL Technologies) supplemented with 1% Penicillin/Streptomycin (Gibco by Thermo Fisher Scientific) and 1% StemSpanTM CC100 (STEMCELL Technologies,) before treatment with either 50 ng/ml BMP2 or BMP4 (R&D Systems #355-BM-010 and #314-BP-010) solved in 0.1% BSA (Sigma-Aldrich by Merck) for 48 h.

Techniques: Labeling